Journal: Stem Cell Research

Article Title: Metabolic maturation of differentiating cardiosphere-derived cells

doi: 10.1016/j.scr.2021.102422

Figure Lengend Snippet: Characterization of cardiosphere-derived cells. ( A) Yield of explant-derived cells and CDCs at P1 and P2 for cells cultured on FN/PDL or ColIV/HD (n = 6). (B) Proliferation of control P2-CDCs on FN or ColIV (n = 3). Data are presented as mean ± SEM and assessed using a two-way ANOVA with Tukey post hoc test ** p < 0.01, *** p < 0.001 compared with P2-CDCs on ColIV for (A) and with P2-CDCs on ColIV at the same time point for (B). (C) Representative flow cytometry dot plots and quantification of flow cytometry analysis of P2-CDCs for CD90 Data are presented as mean ± SEM (n = 3) and assessed using an unpaired t -test. **** p < 0.0001.

Article Snippet: Data were analysed using an unpaired TTest or ANOVA (GraphPad Prism v8.0.1) with a TUKEY multiple comparison as appropriate.

Techniques: Derivative Assay, Cell Culture, Control, Flow Cytometry

Journal: Stem Cell Research

Article Title: Metabolic maturation of differentiating cardiosphere-derived cells

doi: 10.1016/j.scr.2021.102422

Figure Lengend Snippet: Expression of metabolic-related genes in control and 5-Aza treated CDCs. The relative mRNA expression of genes involved in glucose (GLUT1, GLUT4, PDK1 and PDK4) and fatty acid (CD36, CPT1B, PPARα, MCAT and ACADM) metabolism was assessed using qPCR in control and differentiated P2-CDCs created through FN/PDL or ColIV/HD. The mRNA expression was normalized to the geometric mean of Actb and β2M as reference genes and ColIV control as calibrator. Data are presented as mean ± SEM (n = 3), assessed using an ANOVA with Tukey post hoc test. Multicolour stars indicate multiple comparisons between groups and FN and ColIV control, black stars indicate comparison between control and differentiated cells. *p < 0.05; **p < 0.01; ***p < 0.001; **** p < 0.0001.

Article Snippet: Data were analysed using an unpaired TTest or ANOVA (GraphPad Prism v8.0.1) with a TUKEY multiple comparison as appropriate.

Techniques: Expressing, Control, Comparison

Journal: Stem Cell Research

Article Title: Metabolic maturation of differentiating cardiosphere-derived cells

doi: 10.1016/j.scr.2021.102422

Figure Lengend Snippet: Substrate utilisation by control and 5-Aza treated CDCs . (A) The rate of glycolysis and of oxidation of glucose, palmitate and acetoacetate measured in control and differentiated P2-CDCs created through FN/PDL or ColIV/HD. (B) Calculated rates of adenosine triphosphate (ATP) production in control and differentiated P2-CDCs. Data are presented as mean ± SEM (n = 3–5), assessed using an ANOVA with Tukey post hoc test. Multicolour stars indicate multiple comparisons between groups and FN and ColIV control, black stars indicate comparison between control and differentiated cells for A and as indicated for B; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Data were analysed using an unpaired TTest or ANOVA (GraphPad Prism v8.0.1) with a TUKEY multiple comparison as appropriate.

Techniques: Control, Comparison

Journal: Stem Cell Research

Article Title: Metabolic maturation of differentiating cardiosphere-derived cells

doi: 10.1016/j.scr.2021.102422

Figure Lengend Snippet: Metabolic changes after differentiation with TGFβ and WY-14643. (A) The relative mRNA expression of genes involved in glucose (GLUT1, GLUT4, PDK1 and PDK4) and fatty acid (CD36, CPT1B, PPARα, MCAT and ACADM) metabolism was assessed using qPCR in control, and TGFβ differentiated CDCs with and without treatment with WY-14643. The mRNA expression was normalized to the geometric mean of Actb and β2M as reference genes and baseline as calibrator. (B) Changes in rates of glycolysis and glucose and palmitate oxidation, expressed as a fold change over baseline. Data are presented as mean ± SEM (n = 3) and assessed using an ANOVA with Tukey post hoc test. Black stars indicate comparison between baseline and differentiated cells and blue stars indicate comparison with TGFβ differentiated cells; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (C) CDCs differentiated using both TGFβ and WY-14643, stained with Mitotracker® Red CMXRos and showing formation of mitochondrial networks. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Data were analysed using an unpaired TTest or ANOVA (GraphPad Prism v8.0.1) with a TUKEY multiple comparison as appropriate.

Techniques: Expressing, Control, Comparison, Staining